Nanobiosensors have transformed farming through the development of diagnostic techniques, making them essential to agriculture. Nanobiosensors address many environmental and agricultural concerns and are precise and effective. These sensors can be employed to identify fertilizers, moisture content, and various diseases. As a result, plant protectant application may be decreased, crop yields can be improved via effective crop management, and nutrient loss can be minimized. Among the several types, immuno-nanobiosensor is a kind of nanobiosensor that uses a nanomaterial to help detect the creation of an immunocomplex utilizing an antibody or antigen as a bioreceptor. They can identify infections, contaminants, heavy metal ions, and microbial burdens. We review the main approaches used in their creation, such as signal transduction processes, antibody immobilization techniques, and signal amplification tactics based on nanomaterials. The application demonstrates numerous uses of immuno-nanobiosensors for precision agriculture by covering disease detection methods, pathogen surveillance techniques, and pesticide residue testing, alongside soil quality evaluation. We overview the challenges involving the technology’s expenses, repeatability, and practical implementation difficulties. These applications lead to future potential for immuno-nanobiosensors to revolutionize agriculture by improving disease detection and boosting crop productivity while supporting sustainable resource practices.