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The germination of seeds plays a vital role in the propagation and preservation of rare and endangered plant species. This study examines in vitro culture techniques to optimize germination under controlled conditions, focusing on factors critical for rare plants. The key aspects of optimization include disinfection methods; nutrient media; and environmental conditions, namely, moisture, oxygen, temperature, and light. Disinfection techniques, such as sodium hypochlorite and ethanol, effectively reduce microbial contamination while preserving seed viability. Nutrient media such as Murashige and Skoog (MS) and Gamborg’s B5 are optimized for rare plant species. Moisture availability is essential for seed imbibition, and oxygen is vital for respiration and metabolism. Innovations such as hydrogels and controlled-release water maintain optimal moisture and increase transplantation success. Plant growth regulators (PGRs), namely, gibberellins, ethylene, and abscisic acid, are key for overcoming dormancy and promoting germination in rare plants. Oxygen levels influence hormone pathways, and managing reactive oxygen species (ROS) supports seedling development while preventing premature senescence. In summary, optimizing in vitro conditions—moisture, oxygen, and hormonal balance—improves seed germination and supports the conservation of rare plant species, addressing critical biodiversity and sustainability challenges.

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Advancements in In Vitro Seed Germination of Rare, Endangered Plants: Optimization Methods and Environmental Factors

  • 章节

  • 发表日期：

    2025年

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  • Oksana Sytar,
  • Morgan Fays,
  • Julien Miseur,
  • Nicolas Desoignies

摘要

The germination of seeds plays a vital role in the propagation and preservation of rare and endangered plant species. This study examines in vitro culture techniques to optimize germination under controlled conditions, focusing on factors critical for rare plants. The key aspects of optimization include disinfection methods; nutrient media; and environmental conditions, namely, moisture, oxygen, temperature, and light. Disinfection techniques, such as sodium hypochlorite and ethanol, effectively reduce microbial contamination while preserving seed viability. Nutrient media such as Murashige and Skoog (MS) and Gamborg’s B5 are optimized for rare plant species. Moisture availability is essential for seed imbibition, and oxygen is vital for respiration and metabolism. Innovations such as hydrogels and controlled-release water maintain optimal moisture and increase transplantation success. Plant growth regulators (PGRs), namely, gibberellins, ethylene, and abscisic acid, are key for overcoming dormancy and promoting germination in rare plants. Oxygen levels influence hormone pathways, and managing reactive oxygen species (ROS) supports seedling development while preventing premature senescence. In summary, optimizing in vitro conditions—moisture, oxygen, and hormonal balance—improves seed germination and supports the conservation of rare plant species, addressing critical biodiversity and sustainability challenges.