Gelatin nanofibers have received much attention due to their unique features and wide range of applications, particularly in the biomedical and pharmaceutical areas, where they stimulate cell proliferation and adhesion. This research studies the effect of various processing parameters, such as polymer solution content, air pressure, collector distance, and solution temperature on the morphology of Gelatin Curcumin antibacterial nanofibres. This work uses a commercial airbrush air-jet spinning to produce gelatin Curcumin antibacterial nanofibres for potential wound healing applications. The air jet spinning is performed at various processing settings, and a processing window for optimal fibers is provided. It has been observed that when the solution temperature increases, the gelatin curcumin fibers’ length increases, and the fibers’ diameter decreases. Also, the fibers are obtained when the air pressure is maintained at 7–8 bar. Thus, airbrushing with a commercial airbrush is a feasible technology for faster manufacturing of antibacterial nanofibers for wound dressing and scaffold application. This revolutionary approach opens the door to novel materials with better functionality for tissue engineering, medicinal applications, antibacterial use, and scaffolds. These antibacterial nanofibers represent very promising materials for antibacterial applications.