To evaluate the various performance characteristics of a diesel engine fueled by different mixes and pure diesel while operating at a steady pace, an experimental inquiry was conducted. Biodiesels are obtained from triglycerides of vegetables through a transesterification reaction. The biodiesel from Linseed and Karanja vegetable oils is used in this experimental study. An experiment has been carried out to inspect the variation of different performance values of the engine when it is fueled with the different blends of Linseed and Karanja biodiesel. The findings suggest that the performance parameters of the blends closely resemble those of pure diesel. Furthermore, better performance metrics are obtained when a larger concentration of Karanja oil—as opposed to Linseed oil—is added to the diesel mixes. Specifically, the statistics show that blend K15L5D80 outperforms all the other blends, performing almost exactly like diesel alone in diesel engines across a range of characteristics. It’s important to note that some mixtures function almost exactly like pure diesel. It implies that certain blends of biodiesel can function as workable substitutes without sacrificing engine performance, which is necessary for sustainable energy transitions. This effort involves predicting a model through ANN and evaluating the performance of the Diesel engine that is fed with various ratios of conventional fuel and a blend of Linseed and Karanja oil.