Lignin, a natural polymer found in the cell walls of plants, represents an abundant and renewable resource with significant potential for various applications. This chapter examines the novel application of lignin in three-dimensional printing, an area rapidly progressing towards sustainable manufacturing practices. By integrating lignin into three-dimensional printing materials, we can reduce reliance on petroleum-based polymers, enhance mechanical properties, and improve thermal stability of printed objects. Furthermore, lignin contributes to the circular economy by facilitating waste reduction, promoting biodegradability, and enhancing energy efficiency in the production process. However, the integration of lignin into three-dimensional printing materials presents technical challenges, including issues of compatibility and printability that must be addressed to fully realize its potential. This research highlights lignin’s potential to transform three-dimensional printing by encouraging ecofriendly innovation while addressing the challenges of material integration.