Strontium exhibits better bone formation and osteoprotective effects. Importance of the current research is to evaluate in vitro studies of strontium oxide doped borosilicate glass. Glass samples were fabricated by melt quenching method. In vitro bioactivity studies also defined through SBF at 37 ℃ for the time duration of 0, 7, and 14 days. Hydroxyapatite formation was determined using X-Ray Diffraction, Fourier Transform InfraRed Spectroscopy, and Field Emission Scanning Electron Microscopy for structural, functional, and morphological studies. From the above characterizations, it was confirmed that glass shows amorphous nature before immersion in the fluid. Hydroxyapatite layer formation resulted in crystalline form after immersion for 7 and 14 days. Borosilicate bioactive glasses result in low crystallization and comparable bioactivity. According to the research, by doping BG with Sr ions, 2 mol% can allow for a tailored and continuous ion release, increasing the glass's potential use as a functional biomaterial for applications involving bone regeneration.