With China vigorously promoting the new development pattern of “double circulation at home and abroad”, the demand for domestic and international trade has increased significantly, and the hinterland supply transportation mode operated by a single port can no longer meet the increasing transportation demand. At present, it is particularly important to realize increasing profits and higher quality development of ports, and how to achieve good competition and cooperation between ports on this basis. Therefore, based on Bertrand’s model, this paper studies the influence of government subsidies on the competition strategy price and profits of the two ports, and takes Dalian Port and Vladivostok as examples for empirical analysis and parameter change simulation. The results show that the cooperation between the two ports can obtain higher expected returns than mutual competition. Finally, according to the research results, the countermeasures of constructing cooperation mechanism and suggestions of port differential positioning are put forward.