Viral hepatitis is a global health issue that causes liver inflammation and can lead to acute or chronic liver problems if not treated effectively. Early detection of viral hepatitis is crucial for medical intervention, virus control, and antiviral medications, which might be helpful to improve patient outcomes. Different genomic structures are found in hepatitis viruses, including hepatitis A virus (HAV), hepatitis B virus (HBV), hepatitis C virus (HCV), hepatitis D virus (HDV), and hepatitis E virus (HEV), that affect their pathogenicity and epidemiology. Different nucleic acid amplification-based techniques are available for the detection of hepatitis viruses with high specificity and sensitivity. Fluorescence in situ hybridization (FISH) can detect hepatitis A, B, and C viruses in tissues. Moreover, non-amplification-based molecular diagnostics, like next-generation sequencing (NGS) and enzyme-linked immunosorbent assay (ELISA), provide alternative approaches for detecting sequence viral genomes and viral proteins, respectively. Recent clustered regularly interspaced short palindromic repeat (CRISPR)-based systems offer advanced diagnostic capabilities for the quick detection and management of viral hepatitis viruses specially HCV and HBV with high sensitivity, and specificity which have the potential to dramatically improve healthcare system. This book chapter summarizes the recent advancement in the different techniques which are being used or in progress to be used for the detection of hepatitis viruses.