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The disk diffusion method is one of the oldest and most widely used techniques for sensitivity testing, especially in clinical laboratories working in a resource constrained environment. This method is not only cost-effective, but also provides consistent results that are easy to interpret, making it a valuable tool in antimicrobial resistance studies (Berkow et al, Clin Microbiol Rev 33:e00069-19, 2020). In this method, paper disks, each infused with a specific concentration of an antifungal drug, are placed on the surface of an agar plate that has been inoculated with the fungal isolate. Once the disks are attached, they are incubated under optimal conditions to allow fungal growth and diffusion of the drug. After incubation, the size of the clear area around each disk, known as the zone of inhibition, is measured. This zone indicates the extent of drug diffusion through the agar and reflects the isolate’s susceptibility to the antifungal agent tested. A larger zone usually indicates higher susceptibility, while a smaller or absent zone indicates resistance. The Clinical and Laboratory Standards Institute (CLSI) has established standardized protocols for the disk diffusion assay, particularly for yeast susceptibility testing (2nd edition CLSI document M44-A2, 2009). This ensures the reliability and reproducibility of results in different laboratories. In addition, the disk diffusion method enables the simultaneous testing of several antifungal agents, providing a comprehensive understanding of the resistance profile of an isolate. In addition to its practical application in the clinical field, the disk diffusion method is also an indispensable tool for researchers working on fungal pathogens. As this method provides a clear visual representation of susceptibility, it helps to monitor emerging resistance patterns and ultimately contributes to improved patient care and treatment strategies for fungal infections (Sanchez et al, Microbiol Res 252:126867. 2021). Overall, the disk diffusion method remains a cornerstone of antifungal susceptibility testing for fungi and bridges the gap between research and clinical application.

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Disk Diffusion Assay for Testing the Drug Sensitivity of Pathogenic Fungi in Agar

  • 章节

  • 发表日期：

    2025年

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  • Ritu Pasrija,
  • Deepika Kumari

摘要

The disk diffusion method is one of the oldest and most widely used techniques for sensitivity testing, especially in clinical laboratories working in a resource constrained environment. This method is not only cost-effective, but also provides consistent results that are easy to interpret, making it a valuable tool in antimicrobial resistance studies (Berkow et al, Clin Microbiol Rev 33:e00069-19, 2020). In this method, paper disks, each infused with a specific concentration of an antifungal drug, are placed on the surface of an agar plate that has been inoculated with the fungal isolate. Once the disks are attached, they are incubated under optimal conditions to allow fungal growth and diffusion of the drug. After incubation, the size of the clear area around each disk, known as the zone of inhibition, is measured. This zone indicates the extent of drug diffusion through the agar and reflects the isolate’s susceptibility to the antifungal agent tested. A larger zone usually indicates higher susceptibility, while a smaller or absent zone indicates resistance. The Clinical and Laboratory Standards Institute (CLSI) has established standardized protocols for the disk diffusion assay, particularly for yeast susceptibility testing (2nd edition CLSI document M44-A2, 2009). This ensures the reliability and reproducibility of results in different laboratories. In addition, the disk diffusion method enables the simultaneous testing of several antifungal agents, providing a comprehensive understanding of the resistance profile of an isolate. In addition to its practical application in the clinical field, the disk diffusion method is also an indispensable tool for researchers working on fungal pathogens. As this method provides a clear visual representation of susceptibility, it helps to monitor emerging resistance patterns and ultimately contributes to improved patient care and treatment strategies for fungal infections (Sanchez et al, Microbiol Res 252:126867. 2021). Overall, the disk diffusion method remains a cornerstone of antifungal susceptibility testing for fungi and bridges the gap between research and clinical application.