The primary objective of the current study is to develop an experimental setup and conduct an analysis of ignition delay in marine diesel engines under varying hot surface temperatures and air pressures, employing both pure diesel and blended oil. The considered variable parameters include ambient pressures (AP), hot surface temperatures (HST), and injection pressures (IP). An experimental setup was constructed for this purpose, utilizing an optical method to measure ignition delay. The delay in the ignition of the fuel spray was determined by recording the time lapse between the injection event and the onset of flame appearance inside the combustion chamber using a Scope Meter. The results indicate that ignition delays are reduced when using blended fuels compared to pure diesel. The maximum delay in the case of a blend is 10.2 ms, whereas in the case of pure diesel, it is 19.9 ms, corresponding to minimum IP, minimum HST, and minimum AP. Consequently, ignition delay with pure diesel is greater than that with blended fuel. The outcomes of this study are expected to offer valuable insights for marine application designers.