This study is devoted to the production of cellulose from Medicago sativa (Lucerne), as well as the study of its chemical and physicochemical properties. The aim of the work is to develop effective methods for obtaining cellulose and assess its potential for use in various industries. During the experiment, traditional chemical and physical methods such as acid hydrolysis, alkaline treatment and bleaching were used. The obtained cellulose samples were analyzed using IR spectroscopy, gravimetric and visual methods to determine the cellulose content and its purity. The results showed that Medicago sativa (Lucerne) cellulose has good physicochemical properties suitable for further use in biotechnology, the production of biodegradable materials and sorbents. Prospects for application include use as a raw material for the production of nanocellulose, which opens up new opportunities in the field of ecotechnology, medicine and materials science. The work also considers the environmental and economic aspects of using Medicago sativa (Lucerne) as a raw material for cellulose.