This paper presents a structural health assessment system based on a dedicated Printed Circuit Board (PCB) using Micro Electronic Mechanical System (MEMS) accelerometers. Frequency is often considered to be an ideal safety indicator for assessing the status of bridge health. Based on vibration measured from the dedicated PCB, bridge vibration frequency can be identified. The system was first validated with two digital tri-axial accelerometers ADXL345 and ADXL355 on a steel beam at the laboratory at the University of Transport Technology (UTT). Collected data were then transferred to the computer for identifying natural vibration frequencies of the bridge modal. The obtained results were compared to conventional devices based on dynamic strainmeter, servo type accelerometer and simulation model using finite element method. After that, the system was carried out on the Dong Son concrete bridge. In both scenarios, the structural health assessment system ensures accuracy and sensitivity and reliability of the designed PCB in comparison with commercial devices.