This chapter introduces hyperspectral camera (HSC) fluorescence imaging to detect heterogeneous events concerning fluorescent organic molecular crystals. We have achieved the molecular assembling process including the crystallization probed by the fluorescence changes in real time. The heterogeneity of the deposited solids or spatial-dependent evaporation process is exhibited due to the weak intermolecular interactions in the organic molecular aggregation. HSC is a camera equipped with spectroscopic information in each pixel, which enables space-resolved spectroscopy. Examples of HSC fluorescence imaging we observed are present; heterogeneity of the fluorescence doped in the polymer films, molecular domino transformation, evaporative crystallization process, and polymorph formation process. It is found that the survival time of the intermediates such as the liquid-like cluster is important to the destination of the depositions based on the time- and space-resolved fluorescence measurements using the HSC reveal.