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We have found that lysozyme crystallization is promoted by gap-mode surface plasmon resonance of gold nanoparticles (AuNPs). Crystallization experiments were performed by dropping a metastable lysozyme solution in a supersaturated state in which spontaneous nucleation does not occur onto substrates prepared by dropping a 40 nm diameter AuNPs colloidal solution onto a glass substrate and subsequent drying. These substrates were capable of inducing gap-mode surface plasmon resonance. Crystals were deposited on the prepared substrates in numbers that increased with light irradiation, whereas none were deposited on control substrates. A crystallization mechanism involving lysozyme adsorption on AuNPs was proposed. The results showed that the number of lysozyme molecules adsorbed on the AuNPs corresponded well to the number of deposited crystals. We demonstrated that when gap-mode surface plasmon resonance is induced, the enhanced electric field in the gap between the AuNPs traps and concentrates the adsorbed lysozymes, leading to crystallization.

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Surface Plasmon Resonance Induced Nucleation of Protein

  • 章节

  • 发表日期：

    2025年

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  • Tetsuo Okutsu,
  • Taku Yasue,
  • Miku Murakami,
  • Asuka Ito,
  • Tomohiko Sato,
  • Ryoya Nakayama,
  • Tomoka Takasuka,
  • Yoshikiyo Hatakeyama,
  • Soshi Shiraishi,
  • Tatsuya Shoji,
  • Yasuyuki Tsuboi,
  • Yutaka Kasuya,
  • Masahiro Ito,
  • Katsuya Tenjitsu,
  • Akihiro Takura,
  • Hiroaki Horiuchi

摘要

We have found that lysozyme crystallization is promoted by gap-mode surface plasmon resonance of gold nanoparticles (AuNPs). Crystallization experiments were performed by dropping a metastable lysozyme solution in a supersaturated state in which spontaneous nucleation does not occur onto substrates prepared by dropping a 40 nm diameter AuNPs colloidal solution onto a glass substrate and subsequent drying. These substrates were capable of inducing gap-mode surface plasmon resonance. Crystals were deposited on the prepared substrates in numbers that increased with light irradiation, whereas none were deposited on control substrates. A crystallization mechanism involving lysozyme adsorption on AuNPs was proposed. The results showed that the number of lysozyme molecules adsorbed on the AuNPs corresponded well to the number of deposited crystals. We demonstrated that when gap-mode surface plasmon resonance is induced, the enhanced electric field in the gap between the AuNPs traps and concentrates the adsorbed lysozymes, leading to crystallization.