The unique topic of deoxyribonucleic acid (DNA) nanotechnology can be guided and advanced by the multidisciplinary subject that involves the advancement of physics, chemistry, biology, mathematics, engineering, and materials science. For several decades that have passed since Nadrian Seeman’s initial suggestion of DNA origami, remarkable progress has been at a center of interest for development to healthcare applications. In living systems, genetic data is stored and transmitted via the fundamental molecule, recognized as DNA. DNA nanotechnology extracts this molecular information from its biological environment and utilizes its data to put together structural motifs and then fabricate or link them together. This area of study and research has significantly evolved nanotechnology and nanoscience and improved our understanding of molecular self-assembly over decades.