Kuala Lumpur aimed to enhance its public transportation system to increase convenience and accessibility, particularly within the public train network. Understanding pedestrian mobility became critical for developing effective transportation policies, improving user satisfaction, and encouraging the transition to public transport to reduce private vehicle use. This study investigated walking speeds on rail terminal staircases by gender during peak and non-peak hours. The Semi-Automatic Video Analyzer (SAVA) was used for data analysis, with footage recorded at the Masjid Jamek LRT Station. Malaysians were identified by their slower walking speeds—59 m per minute on average compared to Singaporeans, who reached 74 m per minute, despite sharing similar equatorial climates. Data obtained through SAVA were validated using statistical analysis via SPSS software, revealing that gender and peak times influenced walking speeds.