Ageing significantly influences the durability and performance of asphalt mixtures, making them vulnerable to cracking and deformation. To assess the ageing properties of asphalt mixtures, it is best to determine their binder performance. This paper offers a thorough analysis of the ageing characteristics and resistance of asphalt binders, specifically focusing on the incorporation of waste cooking oil (WCO) alongside Reclaimed Asphalt Pavement (RAP). The study employs a series of physical and rheological tests, as well as penetration, softening point, ductility, and dynamic shear test, to evaluate the degree of ageing exhibited by virgin, aged, and rejuvenated aged asphalt binders. The findings indicate that virgin asphalt binder demonstrates the highest degree of asphalt binder ageing, followed by the rejuvenated asphalt binder and aged asphalt binder, respectively. This result suggests that the additional of RAP and WCO serves to improve the asphalt binder’s ageing resistance. This is because the RAP binder is already aged, and WCO possesses an antioxidant that increases the asphalt ageing resistance. By utilising sustainable materials such as RAP and WCO in road construction materials, this research offers valuable insight into improving the longevity and performance of road construction materials by utilizing waste-derived resources in infrastructure development.