Road markings are crucial elements for ensuring safety and guidance for motorists and pedestrians alike. Traditional methods of road marking often involve paints and thermoplastics, which may degrade over time due to environmental factors and heavy traffic. The utilization of glow-in-the-dark materials in road safety construction offers numerous benefits, including enhanced visibility, increased safety, energy efficiency, longevity, versatility, compliance with regulations, and improved emergency preparedness. These advantages make glow-in-the-dark materials a valuable addition to transportation infrastructure projects aimed at promoting safer and more sustainable roadways. This study aimed to investigate and develop reflective asphalt markers using the mixture of epoxy resin and sparkle stones. The mixture ratios that the researchers used were 50% epoxy resin – 50% sparkle stones, 40% epoxy resin – 60% sparkle stones, 30% epoxy resin – 70% sparkle stones. The asphalt marker specimens were tested based on their adhesion, gradation, and reflectivity properties. The study of various epoxy resin and sparkle stones ratios in the production of reflective asphalt markers demonstrates that these materials have a significant influence on the physical properties of the product. The ideal mixture of 30% epoxy resin – 70% sparkle stones create a balance between better reflective and adhesion properties.