A study on the moss flora of the Eastern Ghats, has documented the occurrence of eleven taxa of the genus Fissidens Hedw., namely Fissidens intromarginatulus Bartr., Fissidens pulchellus Mitt., Fissidens sylvaticus Griffith., Fissidens taxifolius Hedw., Fissidens crenulatus Mitt., Fissidens crenulatus var. tityalyanus (Muell. Hal.) Gangulee, Fissidens ceylonensis Doz. and Molk., Fissidens orishae Gangulee, Fissidens flaccidus Mitt., Fissidens bryoides Hedw. and Fissidens bryoides Hedw. subsp. schimidii (Müll. Hal.) Nork. These taxa are distributed across ten localities of Odisha (Kalinga, Kantia, Berbera-Rajin, Buduli, Orchidarium, Kollah, Gurguria Research Station, Lanjighosra, Bureakhata, Joranda Falls), five in Andhra Pradesh (Katiki Waterfalls, Bhairav Kona, Sunkarimetta, Galikonda, Rajeev Gandhi Wildlife Sanctuary), seven in Tamil Nadu (Kolli Hills, Kalrayan Forest Reserve, Bodamalai Forest Reserve, Javadi Hills, Sirumalai Hills, Yercaud, Rose Garden and one locality in Karnataka region i.e. Malai Mahadeshwara Wildlife Sanctuary. Among these, Fissidens pulchellus is newly reported from the eastern Ghats. The species exhibit diverse ecological preferences, with eight being terrestrial, four growing on rocks, and three as epiphytes. This study underscores the importance of remote sensing technologies, such as high-resolution satellite imagery and vegetation indices, for mapping moss habitats and identifying regions with favorable microclimatic conditions. Such interactive approaches can significantly enhance the understanding and conservation of Fissidens species in the Eastern Ghats.