As oilfield development enters the stage of medium to high water content, the corrosion phenomenon of sulfate reducing bacteria (SRB) is becoming increasingly prominent, posing a threat to oilfield production. Although traditional chemical sterilization and sulfur removal methods have certain effects, SRB is prone to drug resistance and may cause secondary pollution, resulting in high costs. Therefore, its application is limited to some extent. In recent years, biological sulfur removal technologies based on the principle of microbial competition and symbiosis have gradually emerged. This article successfully cultivates bacterial strains that can effectively remove sulfur in high-temperature environments and develops corresponding nutrients. This inhibition system is not only resistant to high temperatures, but also has strong activity, with an inhibition rate of over 85% on SRB. More than 300 oil wells were applied on site, and the results showed that this technology effectively suppressed the growth of SRB in the oil wells, significantly reduced the content of sulfides, eliminated the safety risks caused by hydrogen sulfide, and significantly slowed down the corrosion rate of the wellbore.