Q12 in Qinghai Oilfield is a thick glutenite reservoir with relatively stable initial liquid production. Since 2013, reservoir water production has intensified, oil wells have been flooded rapidly, and crude oil production has declined rapidly. In order to control the increase of water cut in reservoirs, it is necessary to carry out in-depth research on profile control and displacement technology in combination with reservoir conditions. A salt-resistant gel is preferably obtained, the microsphere with good compatibility with the stratum is prepared, the gel formula is 0.3% of polymer, 0.15% of crosslinking agent A and 0.3% of crosslinking agent B, the viscosity of the prepared solution is less than or equal to 50 mPa S, the initial gelling time is 4–7 h, and the final gelling strength is 1000 to 10,000 mPa S. The core test shows that the gel has a strong starting ability for residual oil in the low permeability layer of the heterogeneous reservoir. The expansion ratio of the polymer microspheres is 3–4, and the salt tolerance is 180,000 mg/L. Core experiments show that the injection of the microspheres after water flooding can further enhance the recovery of the sand pack model by 12 percentage points. The core displacement test was carried out by using gel and microsphere, and the injection of gel could continue to increase the recovery factor by 10% after the end of water flooding, and the microsphere could further increase the recovery factor by 10% after the weak gel flooding. The alternate injection of gel and microsphere is superior to the single injection system in improving the recovery value. The research results show that the system has a broad application prospect in Q12 reservoir, and has a certain reference significance for the progress of profile control and flooding technology of glutenite in China.