Nowadays, Selective Laser Sintering (SLS) has gained a lot of interest in membrane fabrication; however, the high cost and increasing amount of waste materials have created demands to recycle the used polymer powder. This study aimed to investigate the feasibility of 3D printed polymer membranes fabricated from recycled powder, focusing on the oil–water separation application. The composition of polyamide 12 (PA-12) powder for virgin powder was 100% fresh material while the recycled powder consisted 100% reused materials, up to 20 cycles. Membranes were fabricated using SLS with controlled printing parameters. Virgin and recycled membranes’ surface morphology was visualised using Scanning Electron Microscope (SEM); surface roughness and contact angle of the membrane’s surface were measured. The recycled membranes recorded 9% higher surface roughness values than the virgin membrane, resulting in greater water contact angle of water on the membranes’ surface due to the enhance hydrophobicity. In addition, this investigation also discovered that the top and bottom surfaces have varying roughness contributes to different wettability. It can be concluded, 3D printed polymer membrane using recycled powder is a cost-effective and eco-friendly option for preventing material waste, owing to its superior wettability compared to virgin materials.