The present paper highlights the use of muscle activity studies (EMG) for improving the design of surgical gloves, inspired by surgeons having muscle fatigue whilst using gloves, typically at the base of the thumb. The glove was suitably modified, and it was sought to verify the effectiveness of gloves after modification by experimentation. The particular motions and the associated muscles of the hand were identified. Subjects belong to various age groups, and both sexes were identified. The muscle activity (Basmijan and De Luca in Muscles alive. Williams & Wilkins, 1985) of the subjects for the muscles selected was studied and recorded using appropriate instrumentation for activity before wearing a surgical glove, whilst wearing modified and unmodified surgical gloves and after removing the surgical glove. An analysis system was developed to give qualitative and quantitative indications of muscle fatigue.