This study employs a unique methodology built on statistical analysis of variance to produce and assess hybrid fibre (Sisal + glass + Hemp)/natural fibre composites that capitalize on the characteristics of the constituent fibres. The impacts of fibre type (A), which comprised hemp, glass, and sisal; chemical treatment (B); and fibre volume per cent on the mechanical characteristics of composites, like Tensile Strength (TS) and Tensile Modulus (TM), Flexural Strength (FS) and Flexural Modulus (FM), were analysed. Authors used the Response Surface Methodology (RSM) to create a mathematical model of mechanical characteristics. The mechanical qualities were shown to be most impacted by the percentage volume of hybrid fibre, followed by the kind of hybrid fibres, according to statistical analysis. In contrast, the change in chemical treatment has a negligible effect. Therefore, using sodium bicarbonate (NaHCO3) to treat fibres led to the greatest mechanical characteristics of composites at the maximum percentage of hybrid fibre. Finally, when the fibre composition is 85% glass and 15% hemp, the proposed hybrid composite displayed improved qualities related to the earlier composites depend on specific fibre composition.