Well-seismic calibration is the bridge to establish the connection between well logging and seismic data. The accuracy of the calibration results directly affects the subsequent reservoir inversion results and structural accuracy. In coal-bearing strata, not only is the lithology complex, but the coal seams themselves also have strong shielding effects and tuning effects caused by their own thickness. Therefore, well-seismic calibration in coal-bearing strata with multiple coal seams is more complex and challenging. Taking the DJ block on the eastern edge of the Ordos Basin as an example, the Taiyuan Formation in the coal-bearing strata mainly consists of two sets of coal seams, the 5#coal and the 8# coal. The Rayleigh wave and seismic side lobe wave were used for time-depth calibration with the 5# coal and 8# coal seams as marker beds. The calibration shows: (1) When the overall thickness of the 5# coal seam is thin or shows multiple thin interbeds, after calibration, the top boundary of the 5# coal corresponds to the zero phase position of the wave peak. (2) When the thickness of the 8# coal seam is large, the top boundary of the 8# coal seam is calibrated to the maximum wave trough; when the thickness of the 8# coal seam decreases, the top boundary of the 8# coal seam is calibrated to move downward, closer to the zero phase position. (3) Different coal-rock structures in the 8# coal seam do not affect the calibration position of the top surface of the coal seam. In response to the above phenomena, forward simulation is used, and the seismic profile is decomposed with multiple wavelets to identify the comprehensive response characteristics of multiple coal seams and analyze their influencing factors. The analysis results show that the thickness and frequency of the coal seam are the main factors affecting the calibration position of the coal seam. The superposition of reflection interfaces formed by different lithological combinations vertically is also a reason for the non-one-to-one correspondence between seismic reflection interfaces and coal seam tops. This paper systematically analyzes the characteristics and influencing factors of well-seismic calibration in coal-bearing strata with multiple coal seams, providing guidance and reference for improving the rationality and accuracy of time-depth calibration in coal-bearing strata.