In this paper, CMC-g-PGMA was synthesized by free radical polymerization using GMA as functional monomer based on CMC. Then CMC-g-(PGMA/NH2-r-GA) was obtained by the modification of ethylenediamine and glutaraldehyde, respectively. HRP was grafted onto the polymer and fixed on the cover glass and glass microtubules by adsorption crosslinking. The performance of HRP was studied. After HRP was grafted on CMC-g-(PGMA/NH2-r-GA) and fixed on two glass materials, the stability of the enzyme could be maintained, which proved the feasibility of HRP and polymer grafting. HRP grafted CMC-g-(PGMA/NH2-r-GA) was fixed on the cover slide. After the glass plate was activated, the enzyme activity of HRP was improved, and the absorbance at 414 nm was about 0.7. HRP grafted CMC-g-(PGMA/NH2-r-GA) was fixed on the glass microtube wall. The enzyme activity remained basically unchanged during continuous catalysis for 2 h, and the average absorbance at 414 nm reached about 0.71.