The genus Bidens belongs to the Asteraceae family of flowering plants comprising approximately 230 species that are found across the globe. These plants are known by various common names such as beggarticks, tickseeds, blackjack, cobbler’s pegs, stickseeds, burr marigolds, Spanish needles, and tickseed sunflowers, which are based on the characteristics of their fruits, many of which are bristly and barbed. Bidens species have gained significant attention in recent years due to their potential therapeutic properties, particularly in the fields of anti-inflammatory and analgesic effects. This chapter presents a comprehensive analysis of the phytochemical composition, anti-inflammatory and analgesic properties, mechanisms of action, preclinical studies, clinical trials, therapeutic applications, safety considerations, and future research directions of Bidens species. The anti-inflammatory effects of Bidens species are manifested through the modulation of key inflammatory mediators and pathways, such as cytokines, prostaglandins, and nitric oxide. These plants have been found to exhibit analgesic properties by inhibiting pain signaling pathways and reducing pain perception. Mechanistic studies have shed light on the intricate pathways through which Bidens species exert their anti-inflammatory and analgesic effects, including the modulation of inflammatory enzymes, receptors, and signaling molecules. These mechanisms provide valuable insights into potential therapeutic targets for the identification of novel anti-inflammatory and analgesic agents derived from Bidens species. Preclinical studies have further confirmed the anti-inflammatory and analgesic activity of Bidens species in various animal models, paving the way for clinical trials to assess their therapeutic potential in humans. The potential therapeutic applications of Bidens species extend beyond inflammation and pain, encompassing a wide range of health conditions such as arthritis, diabetes, and cancer. However, it is crucial to carefully evaluate safety and toxicity considerations to ensure the safe utilization of Bidens species as medicinal agents. Future research directions for Bidens species involve exploring novel extraction methods, identifying specific bioactive compounds responsible for their therapeutic effects, elucidating additional mechanisms of action, and expanding their therapeutic applications in various disease models. Bidens species hold great promise as natural anti-inflammatory and analgesic agents, thus justifying the need for continued research in this domain.