Fluid-conveying tubes are frequently employed as component parts in a variety of systems, such as air conditioning, heat exchangers, solar energy, refrigeration, etc. Aluminium tubes are widely used in fluid conveying applications because of their lightweight, low density, and corrosion resistance. For the dynamic control of those systems, the vibrational characteristics of such tubes must be examined. This work focuses on the influence of discrete mass addition and its location on the dynamic properties of a horizontally positioned clamped–clamped aluminium tube transporting a pulsatile fluid. By conducting experimental modal analysis, the natural frequency of tube with and without discrete mass additions are identified. Operational modal analysis is conducted to extract the influence of internal excitation due to flow pulsation. The present study identifies a stiffness addition phenomenon due to the location of the added mass on the tube. The finding of this study points at a possible vibration control method where desired natural frequency and amplitude of vibration can be achieved by adding point masses at suitable positions at controlled flow velocities.