Non-invasive 40 Hz light flicker stimulation (40 Hz-LFS) has recently received widespread attention as a potential therapeutic approach for Alzheimer's disease (AD). Some studies suggest that abnormal neural activity can lead to the onset of AD, which can be improved by regulating neural activity. Compared with optogenetics driven 40 Hz neural oscillations, 40 Hz-LFS is non-invasive, easy to operate, and has a fast action speed. This study is based on a non-invasive light flicker stimulation platform to investigate the effects of 40 Hz-LFS on the pathological manifestations of AD models in mice. APP/PS1 AD mice were randomly divided into the AD group and the AD+40Hz group, and they were subjected to 7 days of 1-h 40 Hz-LFS or pseudo stimulation per day. The mice's behavioral performance was evaluated by the novel object recognition (NOR) and Morris water maze (MWM) tests, and then the whole-cell patch-clamp experiment was conducted to record the action potentials of granule cells in the hippocampal dentate gyrus, and the corresponding indicators were analyzed. The results show that long-term 40 Hz-LFS can improve the behavioral performance of AD mice and enhance their neural excitability. It is expected to reveal the potential physiological mechanism of improving cognitive function from the perspective of behavioral performance and single-cell neural excitability.