Conventional activated sludgeActivated sludge techniques have trouble breaking down resistant contaminantsContaminants including oilOil, greaseGrease, chemicals, insecticides, and plastics in wastewater. Because they are poisonous, and do not naturally degrade causing other major issues, these pollutants are widespread and pose a substantial threat to aquatic habitats and organisms. EnzymesEnzymes are suggested for the treatment of these resistant contaminantsContaminants as a high-efficiency biocatalystBiocatalysts. The roles and uses of enzymesEnzymes in wastewater treatmentWastewater treatment is the key point of this review. Enzymatic processes in remediating resistant pollutants, the identification and ecotoxicityEcotoxicity assessment of enzymatic transformation productsTransformation products, the impact of different types of enzymesEnzymes and their suppliers, and commonly used enzymatic wastewater treatmentWastewater treatment methods are important factors and will be discussed in this review.