The improvement of sustainability without compromising the affordability of construction materials is critical for today's construction sectors. Concrete is one of the most commonly used construction materials that is prone to cracks. The notion of ‘self-healing’ concrete is used to improve the longevity of concrete by increasing its fracture resistance. In bio-concrete (also known as self-healing concrete), microorganisms immobilized in appropriate carriers promote crack repair by microbially induced calcite precipitation. The current study sought to compare the efficacy of several natural materials that could potentially act as bacterial carriers in self-healing concrete. We examined bentonite clay, rice husk ash, mud brick powder, and coconut pith, which were added separately in quantities ranging from 5 to 25% of the fine aggregate fraction in the concrete mixture. The ureolytic bacteria Lysinibacillus sphaericus AKM01 was utilized in this work. Crack healing was examined using the ‘full-wet’ approach. Concrete with 20% mud brick powder might accomplish visible fracture healing in 26 days. Tests on a universal testing machine proved that the addition of mud brick powder at this percentage did not reduce the compressive strength of concrete.