The proposed study aims to design and simulate an integrated microcantilever within a microchannel to analyze the behavior of two different fluids, acetone and benzene, as they flow through the microchannel. The analysis is carried out using the finite element method, which integrates various physical phenomena, including solid physics, laminar flow, moving mesh dynamics, and fluid–structure interaction. The key objective of this research is to calculate the deflection of the microcantilever under various fluid flow conditions while maintaining a constant fluid flow rate. The novelty of this design lies in its ability to investigate and compare the behavior of acetone and benzene within microchannels. The results obtained from the simulation demonstrate noteworthy findings. Compared to a finite element method-based model that was previously designed and analyzed before the simulation, the study incorporates two different types of cantilevers, namely the T-cantilever and R-cantilever. The maximum deflection observed during the study was 34.23 µm when using organic fluid acetone, accompanied by a maximum pressure of 74.62 Pa. Conversely, the minimum pressure and deflection were recorded for the fluid benzene, with values of 0.85 Pa and 4.85 µm, respectively. Notably, the T-cantilever exhibited the maximum deflection and pressure, while the R-cantilever demonstrated the minimum deflection. This research provides valuable insights into the behavior of acetone and benzene in microchannels, offering a comparative analysis of different microcantilever types. These findings contribute to an understanding of microfluidic systems and may have practical applications in various fields, including microscale sensing and fluid analysis.