Antimicrobial Efficacy and Production Optimization of Chitosan Derived from Black Soldier Fly Pupal Shells Exuviate
摘要
This study explores the potential of using Black Soldier Fly (BSF) pupal shells as a sustainable source for chitosan production. The research investigates the deacetylation of BSF chitin to produce chitosan and evaluating the effects of temperature, NaOH concentration, and reaction time on the yield. The optimal conditions identified were 100 °C, 60% NaOH concentration, and a reaction time of 2 h, resulting in a maximum chitosan yield of 67.65%. Antimicrobial assays demonstrated that the produced chitosan exhibited significant inhibitory effects against bacteria, i.e., E. coli and B. subtilis. The findings suggest that BSF-derived chitosan could serve as a viable alternative to conventional sources, offering both economic and environmental benefits.