This paper studies the effects of using glycerol-based deep eutectic solvent (DES) on coconut mesocarp (CM) for lignocellulose pulping. In this study, glycerol-based DES including potassium carbonate-glycerol (PC-Gly), choline chloride-glycerol (ChCl–Gly), betaine-glycerol (BET–Gly), zinc chloride-glycerol ( \({\text{ZnCl}}_{2}\) –Gly) and aluminum trichloride hexahydrate-glycerol ( \({\text{AlCl}}_{3}\) –Gly) was used to extract cellulose from coconut mesocarp. The viscosity, pH of the DES, physical appearance of DES pulp and the cellulose yield were compared accordingly, after the pretreatment process. It was found that the synthesized PC–Gly DES showed the highest viscosity and pH values of 9840 cP and 12.27, respectively. Meanwhile, the \({\text{AlCl}}_{3}\) –Gly exists in a strong acidic DES and showed an obvious color changes after pretreatment, which indicated the greatest removal of the non-cellulosic part from the CM. The result correlates to the highest cellulose recovery of 48.72% by \({\text{AlCl}}_{3}\) –Gly after the pretreatment process. In fact, all five DES pulps exhibited a higher cellulose yield compared to raw CM, which indicates that all DES systems are efficient in extracting the cellulose from CM, thus promoting green processing for cellulose recovery.