Ageing is a prevalent issue in today’s world. Age-related diseases currently lack effective treatment methods. Mesenchymal stem cells (MSCs) are known to have human longevity properties and could serve to treat age-related diseases. However, usage of MSCs from conventional sources faces various limitations as they lack abundance, have low proliferation rate, and require invasive harvesting procedures. Alternatively, human umbilical cord lining MSCs (CLMSCs) are found to also possess anti-ageing properties and could be better suited for medical use due to their abundance, low immunogenicity, and non-invasive harvest. Nonetheless, the anti-ageing molecular mechanism of CLMSCs remains underexplored and is thus not readily used for the treatment of age-related diseases. Therefore, our study aims to: so that we can understand the anti-ageing molecular mechanism of CLMSCs, and thus be able to safely and effectively utilise CLMSCs in regenerative medicine. In our research, rats were injected with CLMSCs to test for anti-ageing effects and immunohistochemistry was conducted on human umbilical cord tissue to identify biomarkers that could be responsible for the human longevity effects of CLMSCs. Our results demonstrated the anti-ageing effects of CLMSCs and identified the presence of GDF-11 and IL-24 biomarkers in human umbilical cord lining and vessels, which are rich in CLMSCs, and likely confer CLMSCs its human longevity properties. We conclude that CLMSCs from human umbilical cord tissue have great potential for use to prevent and cure age-related diseases.