This chapter provides an in-depth exploration of the transformative potential of Industry 5.0 on operations and the digital supply chain. Building upon the foundational principles of human-centric collaboration and advanced digital technologies, it represents a paradigm shift emphasizing integrating human skills with automation and data-driven insights. Through a comprehensive review of literature, case studies, and industry practices, this paper elucidates the key components, benefits, challenges, and future trends associated with revolutionizing Industry 5.0 operations and the digital supply chain. Moreover, it equips organizations with practical insights and recommendations, giving them the confidence and readiness to leverage Industry 5.0 principles to enhance competitiveness, agility, and sustainability in an increasingly digitalized business landscape.