Cruciferous vegetable crops constitute the largest group that is consumed in raw, cooked, and processed forms. These vegetables provide nutrients, minerals, and other allelochemicals important for strengthening our immune system. Breeders and researchers are continuously working on cultivar development and improving particular hybrids to exploit heterosis. Hybrid breeding is cumbersome and is a labor-intensive operation due to the manual pollination of female plants. In this situation, male sterility (MS) provides a workable solution to the breeder to minimize labor expenses and reduce operational time. Two different MS systems are exploited in hybrid breeding: cytoplasmic male sterility (CMS) and genic male sterility (GMS). Cruciferous vegetable crops are cross-pollinated and display significant heterosis. In these crops, the CMS and GMS resources have been identified and widely exploited for hybrid breeding. Some crucifers lack endogenous CMS; therefore, Ogu-CMS has been successfully transferred to these crucifers for hybrid breeding. In this chapter, we briefly discuss the CMS and GMS resources so far identified and utilized in cruciferous vegetables and their genetic and molecular mechanism.