The induction of male sterility in crop plants is vital in reducing the cost of hybrid seed production. The genic or nuclear, cytoplasmic and cytoplasmic-genic male sterility systems have been exploited commercially across the vegetable crops. Despite their popularity and significant advantages, practical achievements are meagre in the case of tomatoes via male sterility systems. However, more than 55 male-sterile mutants have been recorded in tomatoes, and some of them have been investigated extensively at the genetic and genomic levels to explore their suitability in hybrid breeding. Some of the mutants have problems with stability under varying environmental conditions, which limits their use. Recently, genome engineering via the clustered regularly interspaced palindromic repeats-CRISPR-associated protein 9 (CRISPR/Cas9) system has revolutionized plant science research, and novel male-sterile lines have been generated in tomatoes through this potential approach. This chapter highlights the current status of various male-sterile mutants in tomatoes, their role in hybrid breeding, and the applications of genetic and genomic tools, including transgenics, genome editing, and ribonucleic acid interference (RNAi), in expediting tomato hybrid breeding.