This chapter explores the influence of high-throughput technologies on enhancing the biological control of insect pests. Given the criticism of the environmental and health effects of traditional pest control methods, high-throughput techniques offer alternative options for pest management. This chapter provides an overview of various high-throughput methods, such as genomics, metagenomics, and phonomics, which facilitate rapid identification and investigation of biocontrol agents and their interactions with pests. This chapter also addresses significant concerns such as the absence of robust data analysis frameworks and the failure to incorporate genetic data into practical applications. Furthermore, this chapter explores potential methods to improve the efficacy and efficiency of biological control tactics by utilizing these technologies, such as developing targeted interventions and optimizing the microbial communities associated with biocontrol organisms. In this chapter, the authors provide a comprehensive overview of how the integration of high-throughput technologies and biological control might enhance pest management and promote agricultural sustainability.