3D printing is a different term for additive manufacturing. Since the early 1980s, it has been utilized in the automobile and aviation sectors. By using additive manufacturing in practice, it is possible to build prototypes of high-volume industrial parts for testing purposes and the necessary geometries in order to optimize materials and costs. Using thermoplastic materials that are extruded, FDM technology is one of the most comprehensive forms of additive manufacturing. When using FDM manufacturing, the best parameter selection greatly affects the printed part's mechanical characteristics and print quality. Lattice structures are repeating patterns that, when connected, form three-dimensional shapes. They are employed in 3D printing to create lightweight structures with complex geometries that would be difficult or impossible to produce using traditional manufacturing methods. Apart from weight reduction, they are also used to improve the strength, energy absorption, heat transfer, reduce warping and printing time etc. Research on employing lattice structure for aerospace, automotive, medical devices has been extensively carried out currently due the benefits that it offers. Among the various additive manufacturing techniques, Fused Filament Fabrication (FFF) 3D printing is one of the most applied techniques to polymers and composites in the literature. The development of fibre-reinforced composites using FFF has enhanced the mechanical properties of 3D printed polymeric parts. In this investigation, lattice structure of PLA with addition of wood particles and two printing orientation of 0° degree and 90° degree were printed. In this study, tensile and flexural properties and vibration tests were carried out according to the standard. For vibration test, cantilever mode is studied for finding the natural frequency and damping factor of the printed part. From the results, it is concluded that the 0° orientation part has the highest tensile properties, flexural properties i.e. 100.65 MPa and 287 MPa which 5% and 25% higher than the 90° orientation. Also, the tensile and flexural modulus of the 0° orientation is 10.5 GPa and 34.41 GPa respectively. Similarly, the natural frequency of 0° orientation is 63.48 Hz in (Mode-I) which is around 4% higher than the 90° orientation part which shows that the build orientation is often one of the most influential one.