Lignocellulosic biomass (LCB) encompasses plant-derived organic materials rich in cellulose, hemicellulose, and lignin. With its abundant availability, LCB presents a promising feedstock for diverse applications such as biofuel production, bio-valued products, and animal feed. However, the presence of anti-nutritional factors and microbial inhibitors necessitates careful detoxification before its utilization as animal feed. This chapter delves into the types and roles of hemicellulolytic enzymes, various detoxification methods, and the benefits and challenges of enzymatic detoxification, highlighting the advancements and future prospects in this field. Enzymatic detoxification, particularly using hemicellulolytic enzymes, emerges as a preferred method due to factors like specificity and efficiency. These enzymes enhance the availability of fermentable sugars and remove toxic compounds, improving the digestibility and nutritional value of LCB. Despite challenges in enzyme production costs and feedstock variability, advancements in enzymatic technology are making significant strides. The adoption of these methods not only enhances the quality of animal feed but also supports sustainable agricultural practices by utilizing agricultural residues.