In the Pressure Transient Analysis of reservoirs with significant temperature variations, the flow and temperature fields are coupled rather than independent of each other due to the presence of fluid flow in the pore space and the temperature difference in the rock accompanied by significant heat exchange. At this time, the reliability of Pressure Transient Analysis based on isothermal hypothesis is obviously not enough. In order to solve the above problems, a fluid-thermal coupling well test model was established based on heat transfer and fluid mechanics of porous media and considering the coupling influence of reservoir thermodynamic parameters on the flow field. Multiphysics field coupling simulation and well test study of actual non-isothermal reservoirs were carried out by COMSOL Multiphysics software, and typical well test curves of fluid-thermal coupling were plotted, and curve characterization and parameter sensitivity analysis were carried out for the typical well test curves. The analysis results of typical fluid-thermal coupling well test curves show that the thermodynamic parameters of the rock and reservoir fluid have a certain influence on the dynamic changes of wellbore pressure. The results of the study may provide theoretical guidance on the problem of non-isothermal well test.