Traditional fabrics are not always effective at draining sweat quickly and in a specific direction, which causes an undesired wet adhesion sensation. Asymmetric wettable textiles with hydrophilic and hydrophobic materials distributed on fabric on either side can form a wettability difference, which results in a directional water transfer (DWT) property to improve the above issue. Nowadays, the effect of hydrophilic yarn type and fabric density on the DWT in textiles is unclear. To address this issue, six types of weft-knitted fabrics were developed by using hydrophilic yarns (Cotton, Tencel, and Bamboo Pulp) and the PLA hydrophobic filament, and then the varying density of the fabric was adjusted by the loop length. The properties of the fabrics were evaluated by capillary effect, water absorption, WCA, and accumulative one-way transport capacity (O). The results demonstrated that the fabrics prepared to the various hydrophilic yarns and the PLA display different DWT functions, and the effect is PLA/Cotton > PLA/Bamboo Pulp > PLA/Tencel, whilst the density has a great impact on the DWT behavior. To sum up, the PLA/Cotton fabric with lower density has an O-value of 542.28%, which has an optimal DWT function and can be applied to the summer garment fabric.