Glutenite reservoir is characterized by serious heterogeneity and uneven distribution of pores and throats. The existence of threshold pressure gradient has caused great resistance to the development of glutenite reservoir with low permeability, so it is necessary to study the threshold pressure gradient. Firstly, the cores from the oil field were selected under the condition that whose physical parameters were known, and threshold pressure gradients of the cores with water drive and oil drive were measured respectively. Then, the relationship between threshold pressure gradient of the core with single-phase drive and mobility was obtained by curve fitting method. The experimental and calculation results show that the minimum threshold pressure gradient of water drive is lower than that of oil drive, but the maximum threshold pressure gradient of water drive is higher than that of oil drive, which can be inferred that the reservoir is water wet. In addition, the threshold pressure gradient has a power function relationship with mobility. With the increase of mobility, the threshold pressure gradient first decreases rapidly and then decreases slowly, and the minimum threshold pressure gradient decreases faster than the maximum threshold pressure gradient. In this study, the relationship between threshold pressure gradient and mobility of glutenite reservoir with low permeability is systematically explored, and a method to determine the flow state of single-phase fluid in low-speed non-Darcy flow stage is given, which can provide reference for the study of seepage law in glutenite reservoir.