With the rapid advancement of internet technology, e-commerce has gradually become the preferred shopping method for modern consumers. E-commerce not only provides convenient shopping channels, but also brings consumers a rich and diverse way to choose products. But behind this, an excellent e-commerce interface design and excellent user experience are the keys to success. Human-computer interaction design, as the core to ensure that users can obtain a smooth, intuitive and pleasant experience when using e-commerce platforms, has become the focus of the industry. Through in-depth research and application of human-computer interaction design, this article can not only improve user satisfaction and loyalty, but also effectively increase the conversion rate, thus bringing greater business benefits to the enterprise. Research results show that after using intelligent classification and human-computer interaction, the growth rate of clicks on the e-commerce interface is 18 to 21%, the growth rate of user system entries is 27 to 30%, and the transaction growth rate is 29 to 42%. It can be seen that an in-depth discussion of the application of human-computer interaction in e-commerce interfaces is of great significance in promoting the sustainable development of the e-commerce industry.