To diagnose and understand the reason for numerous pathological complications and physiological abnormalities, the detection of hormones is significant. Voltammetric sensors, known for their sensitive and fast responses and potential for miniaturization, can be used as excellent devices for detecting hormones. This chapter discusses enhancements of new electrochemical sensors’ design and utilization of those sensors in hormone-detecting studies. It examines the unique challenges associated with hormone analysis, such as low concentration levels and complex biological matrices, and how voltammetric techniques can effectively address these issues. The integration of nanomaterials, molecularly imprinted polymers, and other innovative materials into sensor platforms is examined, highlighting their influence on sensor performance in terms of sensitivity, selectivity, and stability. Various case studies are presented, demonstrating the successful application of these sensors in detecting key hormones such as insulin, cortisol, and thyroid hormones in clinical and environmental samples. The chapter also explores future trends and challenges, including the requirement for on-the-fly examination and the development of portable and wearable sensor devices. By advancing the capabilities of voltammetric sensors, this work aims to contribute to the broader field of hormone analysis and offer new possibilities for non-invasive diagnostics and personalized medicine.