Prayers are an integral part of wellbeing. This systematic review investigates the link between prayer and wellbeing by examining 87 papers published between 2000 and 2020. The databases utilized include Scopus, Google Scholar, and Web of Science. We applied standardized quality assessment criteria, and independent reviewers selected eight studies for inclusion. The novelty of this study lies in its comprehensive time frame, rigorous selection criteria, and the use of multiple databases, which enhance the reliability and breadth of the review. The present investigation distinguishes itself from existing research by offering a robust synthesis of two decades of studies, ensuring high-quality inclusion through meticulous criteria, and providing a broad yet detailed examination of the connection between prayer and wellbeing. Our analysis reveals a consistent positive correlation between prayer and wellbeing. This research contributes to a holistic understanding of prayer’s positive impact on overall wellbeing and offers practical applications for enhancing mental health interventions, promoting holistic health practices, and informing public health policies. It invites further exploration and application to improve wellbeing at individual and community levels.