In recent years, there has been a growing emphasis on the pursuit of environmentally sustainable energy sources, driven by the urgent need to safeguard our planet and establish a sustainable future. In response to the imperative of securing a sustainable future, this research focuses on the development and optimization of Canna indica-based Plant Microbial Fuel Cell (PMFC) employing carbon cloth electrodes, wastewater as the source of nutrients, and soil serving as the source of electrogens. After a 30-day evaluation period, a peak power density of 81 mW per square meter was achieved. Statistical techniques namely Plackett Burman were used to identify key factors influencing bioelectricity generation, with wastewater pH, electrode surface area, and electrode distance emerging as critical variables. A second-order polynomial equation describing their complex interplay was derived using the Response Surface Methodology, emphasizing the significant interaction between pH and electrode area. These findings provide a solid foundation for the future application of PMFC technology in bioelectricity generation, contributing to the ongoing quest for environmentally sustainable energy sources and a cleaner, eco-friendly energy landscape.