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Protein folding/unfolding is an intriguingly complex process that has been studied for decades now. Atomic force microscopy, optical tweezers, and magnetic tweezers grouped under the umbrella of single-molecule force spectroscopy have complemented our understanding of this complex process by applying mechanical force as a denaturant. The current chapter discusses our previous understanding of the “protein folding problem,” effect of force as a denaturant, and principles and working of the single-molecule force spectroscopy tools and novel insights they provided on the process of protein folding and unfolding.

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Force Spectroscopy Tools for Probing the Mechanochemistry of Protein Folding/Unfolding

  • 章节

  • 发表日期：

    2025年

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  • Pushpkant Sahu,
  • Soham Mukherjee,
  • Jijith Mepperi,
  • Sandipan Saha,
  • Khushboo Goel,
  • Hema Chandra Kotamarthi

摘要

Protein folding/unfolding is an intriguingly complex process that has been studied for decades now. Atomic force microscopy, optical tweezers, and magnetic tweezers grouped under the umbrella of single-molecule force spectroscopy have complemented our understanding of this complex process by applying mechanical force as a denaturant. The current chapter discusses our previous understanding of the “protein folding problem,” effect of force as a denaturant, and principles and working of the single-molecule force spectroscopy tools and novel insights they provided on the process of protein folding and unfolding.