Admixtures are incorporated into concrete to enhance its workability. The setting time of concrete significantly influences its workability. Due to the increasing demand for ready-mix concrete, research for diverse admixtures offering various benefits is crucial. Retarders are generally used to delay the setting time of ready-mix concrete. Organic acids and their derivatives show promise as a viable retarder for ready-mix concrete. Gluconic acid (GA) is an organic acid derived from the oxidation of glucose, can be used to delay the setting time and enhance the workability of ready-mix concrete. This paper presents the results of an experimental investigation conducted to evaluate the potential of gluconic acid as a retarder in concrete. The effect of GA dosages 0.02–0.1% (by weight of cement) on the setting time, workability, compressive strength and water absorption was investigated. The results revealed that addition of 0.1% GA prolonged the setting time and enhanced the workability of concrete without any adverse effect on the strength and durability.